Select No Blank Run Subtraction if no correction is to be performed.
If the absorption values of a specific sample are to be considered for sample evaluation, determine which sample is to be used as Blank Run Sample.
Select Subtract Recent Blank Run Sample in Corresponding Sequence if the Blank Run Sample (sample type: Blank) that was processed last in the current sequence before the current sample is to be used.
Select Subtract a Fixed Sample to perform the correction with a specific Blank Run Sample. Click Browse to search for the sample.
When subtracting a blank run sample, the chromatogram of the blank sample is subtracted point by point from the active chromatogram. If the current sample is a standard sample, the difference between the two chromatograms is used for the calibration.
Enable Matrix Blank Subtraction enables the subtraction of matrix blank samples. Contrary to the other options, the resulting peak areas or peak heights are subtracted for peaks that have been identified for the matrix blank sample and for the unknown sample.
Tip:
Although sample types other than "blank" can be subtracted as well, this usually does not make sense because often-negative peaks would occur in the resulting chromatogram.